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Primary Antibodies  

Rabbit anti-GW182 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (2)
Target:

GW182

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 1225 and 1275

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

GW182 (glycine-tryptophan protein of 182 kDa) is a member of the trinucleotide repeat containing 6 protein family. It functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. GW182 has been found to associate with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibition of GW182 expression delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing [taken from NCBI GeneID:27327].

27327

TNRC6A

trinucleotide repeat containing 6A

Q8NDV7

Trinucleotide repeat-containing gene 6A protein

Alternate Names

CAG repeat protein 26; CAGH26; CTD-2540M10.1; EDIE; EMSY interactor protein; FAME6; glycine-tryptophan protein of 182 kDa; GW1; GW182; GW182 autoantigen; Protein GW1; TNRC6; trinucleotide repeat-containing gene 6A protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.