/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-NO66 Antibody
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Primary Antibodies  

Rabbit anti-NO66 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

NO66

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 50 and 100

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

lysine-specific demethylase NO66 (nucleolar protein 66) specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Additionally, NO66, preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2).

79697

RIOX1

ribosomal oxygenase 1

Q9H6W3

Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66

Alternate Names

60S ribosomal protein L8 histidine hydroxylase; bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66; C14orf169; histone lysine demethylase NO66; hsNO66; JMJD9; lysine-specific demethylase NO66; MAPJD; MYC-associated protein with JmjC domain; NO66; nucleolar protein 66; ribosomal oxygenase 1; ribosomal oxygenase NO66; ROX; up-regulated in lung cancer 2; URLC2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.