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Primary Antibodies  

Rabbit anti-AARS Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (2)
Target:

AARS

Reactivity:

Human

,

Mouse

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 275 and 325

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human,

Mouse

Presumed Reactivity

Golden_hamster,

Rat

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

Alanyl-tRNA synthetase, cytoplasmic (AARS) catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). AARS also edits incorrectly charged tRNA(Ala) via its editing domain [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P49588].

16

AARS

alanyl-tRNA synthetase

P49588

Alanine--tRNA ligase, cytoplasmic

Alternate Names

alanine tRNA ligase 1, cytoplasmic; alanine--tRNA ligase, cytoplasmic; Alanyl-tRNA synthetase; alanyl-tRNA synthetase, cytoplasmic; alaRS; CMT2N; EIEE29; renal carcinoma antigen NY-REN-42

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.