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Primary Antibodies  

Rabbit anti-EpCAM Recombinant Monoclonal Antibody [BLR077G]

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

EpCAM

Reactivity:

Human

,

Mouse

Applications:

Flow Cyt

,

ICC

,

IHC

,

IHC-IF

,

IP

,

WB

Platforms:

COMET™

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR077G

Format:

Whole IgG

Immunogen:

between 264 and 314 (C-term)

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

Product Details

Specifications
Verified Reactivity

Human,

Mouse

Antigen Species

Human

Concentration
500 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


Request Formulation Change

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Additional Product Information

EpCAM, or epithelial cell adhesion molecule, is a type I transmembrane protein expressed on most normal epithelial cells and gastrointestinal carcinomas. It functions as a homotypic calcium-independent cell adhesion molecule. EpCAM is being used as a target for immunotherapy treatment of some human carcinomas [taken from NCBI Entrez Gene (Gene ID: 4072)].

4072

EPCAM

epithelial cell adhesion molecule

P16422

Epithelial cell adhesion molecule

Alternate Names

adenocarcinoma-associated antigen; CD antigen CD326; cell surface glycoprotein Trop-1; DIAR5; EGP; EGP-2; EGP314; EGP40; Ep-CAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; epithelial glycoprotein 314; ESA; hEGP314; HNPCC8; human epithelial glycoprotein-2; KS 1/4 antigen; KS1/4; KSA; M4S1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; TACSTD1; TROP1; tumor-associated calcium signal transducer 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.