System.Dynamic.ExpandoObject
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Purified Human IgG, normal serum

Bethyl Laboratories®

Bethyl Laboratories® Catalog # P80-105

Target:
Human IgG
Host:
Human
10 mg

For ordering information, see our International Distributors

Product Details

Specifications
Antigen Species
Human
Stock Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
2 years from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
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Prod Procedures
Purified normal human IgG is isolated using fractionation and DEAE chromatography.

Protein concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

By immunoelectrophoresis the IgG was shown to 1) react with antiserum specific for human IgG and light chains, 2) not react with antiserum specific for other immunoglobulin classes (IgA, IgM, IgD, IgE), and 3) produce a single precipitin arc with antiserum against human serum identical to that produced with anti-IgG.

By ELISA the IgG contains less than 0.1% of the other immunoglobulin classes.

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Applications

Used as a positive control in immunoassays. Serum or plasma from donor has been tested and found negative for HIV-1 antigen, antibodies for HIV and HCV, and non-reactive for HBsAg, HIV-1 RNA, and HCV RNA. Not tested for Anti-HBc. HANDLE AS IF CAPABLE OF TRANSMITTING INFECTIOUS AGENTS.

Citations (2)