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F(ab')2 Goat anti-Human IgG-F(ab')2 Fragment Cross-Adsorbed Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog # A80-249A

Target:
Human IgG-F(ab')2 Fragment
Reactivity:
Human
Host:
Goat
Clonality:
Polyclonal
Format:
F(ab')2
IsoType:
IgG
Purity:
Antigen Affinity Purified
0.5 mg

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Human
Antigen Species
Human
Minimum Reactivity Species
mouse,rat
Stock Concentration
0.5 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
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Prod Procedures
Antiserum was solid phase adsorbed to ensure specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human IgG-F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

By immunoelectrophoresis and ELISA this antibody reacts specifically with F(ab')2 fragments of human IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with F(ab')2 fragments of IgG from other species.

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Applications

Not all listed applications have been specifically tested by our laboratory.

Citations (1)