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Rabbit anti-ZEB2/SIP IHC Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
ZEB2/SIP
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 750 and 800
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (50+ slides)

10 µl (5+ slides)

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Product Details

Specifications
Verified Reactivity
Mouse, Human
Presumed Reactivity
Ferret, White-tufted-ear marmoset, Crab-eating macaque, Panda, Monkey, Pig, Naked mole rat, Rabbit, Small-eared galago, Little brown bat, Thirteen-lined ground squirrel, Gorilla, Chimpanzee, Northern white-cheeked gibbon, Dog, Bovine, Orangutan, Horse
Antigen Species
Human
Stock Concentration
250 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to ZEB2/SIP immobilized on solid support.

The epitope recognized by IHC-00691-T maps to a region between residue 750 and 800 of human Smad interacting-protein 1 using the numbering given in entry NP_055610.1 (GeneID 9839).

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

Smad interacting protein 1 (SIP1) is a transcriptional inhibitor that binds to the DNA sequence, 5'-CACCT-3', in different promoters. SIP1 has been shown to repress the transcription of E-cadherin. Defects in the gene which encodes for SIP1, ZEB2, are the cause of Hirschsprung disease-mental retardation syndrome (Hirschsprung disease) also known as Mowat-Wilson syndrome (MWS). Hirschsprung disease is a rare autosomal dominant complex developmental disorder [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60315].

9839

ZEB2

zinc finger E-box binding homeobox 2

O60315

Zinc finger E-box-binding homeobox 2

Alternate Names

HSPC082, SIP1, SIP-1, SMAD interacting protein 1, Smad-interacting protein 1, SMADIP1, ZFHX1B, zinc finger E-box-binding homeobox 2, zinc finger homeobox 1b, Zinc finger homeobox protein 1b

Applications

Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections. In some cases, the antibody may be diluted further than indicated.

Citations (1)