Resources

Check out our wide range of resources, including articles, protocols, webinars, videos, posters and documentation.
  • Ushering in the Second Wave of Checkpoint Blockade Therapies
    Immune checkpoint blockade has emerged over the past decade as a useful therapeutic tool against many types of tumors. Checkpoint blockade therapies take advantage of the fact that many tumors are infiltrated by immune cells, but those cells are unable to effectively kill the tumor due to inhibitory signals that limit their function. By reversing inhibitory signals that block T cell functionality, checkpoint blockade therapy reactivates immune cells already in a tumor and allows them to resume their effector function1.
  • Defeating COVID-19: The Science Behind a New ELISA for COVID-19 Seroconversion Detection
    The recent and rapid spread of the novel coronavirus, SARS-CoV-2, has necessitated the development of new assays that are capable of detecting the presence of this virus in patient samples or evidence of recent infection. One strategy being developed are assays to detect the presence of anti-SARS-CoV-2 antibodies in patient sera.
  • No Image
    BRD4: Epigenetic Regulator and Emerging Cancer Target
    Bromodomain protein 4 (BRD4) is a transcriptional regulator that plays a key role in cancer, autoimmunity, and inflammatory diseases1,2. BRD4 was discovered as a protein bound to acetylated chromatin during cell cycle progression. In this way BRD4 maintains consistent gene expression during subsequent rounds of division, a phenomenon known as epigenetic memory or "bookmarking" for gene transcription2,7. BRD4 also plays a critical role in regulating differentiation and development2,4. In the absence of BRD4, bone marrow stem cells are unable to generate B and T cells2. BRD4 is required for the re-expression of stem cell genes during reprogramming of MEFs or B cells to induced pluripotent stem cells2, and also plays a role in osteoblast differentiation4. The role of BRD4 in cell cycle control and differentiation has made it an emerging therapeutic target for cancer and immune system pathologies.
  • Hypoxia Signaling: HIF1-alpha & HIF2-alpha Recombinant Rabbit Monoclonal Antibodies
    The hypoxic response pathway is triggered by low levels of oxygen in the cellular environment. Hypoxia inducible transcription factor (HIF) is central to the hypoxic response. HIF exists as a heterodimeric transcription factor composed of an alpha and beta subunit. In mammals there are three HIF-alpha subunits, HIF1-alpha, HIF2-alpha, and HIF3-alpha, and one beta subunit, the aryl hydrocarbon receptor nuclear translocator (ARNT). The overexpression of HIF1-alpha and HIF2-alpha is associated with poor survival rates for various cancers. Experimental and clinical evidence strongly suggests HIF1-alpha and HIF2-alpha influence tumor development and response to treatment. Because of this, there has been major interest in developing selective HIF inhibitors; but due to the complexity of the HIF pathway, the process has been challenging. Thus, future work for therapeutic targeting of the HIFs will require a better understanding of both the HIF1-alpha and HIF2-alpha pathways.
  • Upgrade Your T-Cell Marker Research With Bethyl Antibodies
    Rapid progress in the field of cancer immunotherapy is a result of advances in our understanding of immune marker expression, antigen presentation, and the cell-mediated immune response involving CD3+ T-lymphocytes. T-cells are crucial to the body’s immune response to cancer, both endogenously and following therapeutic intervention. A T-cell marker panel that can identify subsets of tumor-associated T-cells allows scientists to identify and target subpopulations of T-cells playing unique roles in the immune response against a tumor.
  • No Image
    Tyramide Signal Amplification (TSA)-Based Immunofluorescent Multiplex (mIF) Assays
    Multiplex immunofluorescent (mIF) assays provide information on the expression levels of biomarkers. This protocol gives you the guidelines to optimize immunofluorescence assays.
  • Cell Lysate Preparation - NETN Method
    This protocol outlines cell lysate preparation using the NETN method. This includes the recipes for reagents and the procedures for adherent cells and suspension culture.
  • Cell Lysate Preparation - Nuclear Extract
    Extracts prepared from the isolated nuclei of cultured cells can be used in functional studies and for the purification of proteins. This protocol adapted from Mirmira Lab, University of Virginia outlines the steps and processes required.
  • Cell Lysate Preparation - RIPA Method
    RIPA (Radioimmunoprecipitation Assay) - Buffer is a reagent used in cell lysis experimentation, to enable rapid, efficient solubilization of proteins. By minimizing non-specific protein binding, specific binding interactions can be easily studied and are commonly used in immunoprecipitation experiments.
  • Fixation and Permeabilization of Whole Blood with Red Blood Cell Lysis
    Find out more about the reagents required alongside the cell fixation and permeabilization protocol when dealing with red blood cell (RBC) lysis of whole blood.
  • 2-Step Immunoperoxidase Procedure: Formalin-fixed, Paraffin-embedded tissues and cell blocks
    See the protocol steps detailing the 2-step immunoperoxidase procedure: formalin-fixed, paraffin-embedded tissues and cell blocks method, with the reagents and procedure to successfully complete this experiment.
  • 2-Step Immunoperoxidase Procedure: Epitope Retrieval Method for Formalin-fixed, Paraffin-embedded tissues and cell blocks
    See the protocol steps detailing the 2-step immunoperoxidase procedure: epitope retrieval method for formalin-fixed, paraffin-embedded tissues and cell blocks method, with the reagents and procedure to successfully complete this experiment.
  • 2-Step Immunoperoxidase Protocol: Formaldehyde-Fixed Cells and Cytospin Preparations
    This 2-step immunoperoxidase protocol should be used for formaldehyde-fixed cells and cytospin preparations. Includes the required reagents, preparation of reagents and the step-by-step procedure outline.
  • 2-Step Immunoperoxidase Protocol: Cells Grown in Culture and Cytospin Preparations
    This 2-step immunoperoxidase protocol should be used for cells grown in culture and cytospin preparations. Includes the required reagents, preparation of reagents and the step-by-step procedure outline.
  • 2-Step Immunofluorescence Protocol: Formalin-fixed, Paraffin-embedded tissues and cell blocks
    See the 2-step immunofluorescence protocol to be used for formalin-fixed, paraffin-embedded tissues and cell blocks. Includes required reagents, preparation steps and procedure.