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F(ab')2 Donkey anti-Rabbit IgG-Heavy and Light Chain Cross-Adsorbed Antibody DyLight® 650 Conjugated

Bethyl Laboratories®

Bethyl Laboratories® Catalog # A120-216D5

Target:
Rabbit IgG-heavy and light chain
Reactivity:
Rabbit
Host:
Donkey
Clonality:
Polyclonal
Format:
F(ab')2
IsoType:
IgG
Conjugate:
DyLight® 650
Purity:
Antigen Affinity Purified
0.5 mg

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Rabbit
Antigen Species
Rabbit
Minimum Reactivity Species
bovine,chicken,goat,human,mouse,rat,sheep
Stock Concentration
0.5 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
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Prod Procedures
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 650.

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, human, mouse, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.

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Applications

Not all listed applications have been specifically tested by our laboratory. DyLight® 650 is excited at 652 (in PBS) and emits at 672 (in PBS). DyLight® 650 replaces DyLight® 649. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.