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F(ab')2 Goat anti-Human IgG-F(ab')2 Fragment Antibody FITC Conjugated

Bethyl Laboratories®

Bethyl Laboratories® Catalog # A80-149F

Target:
Human IgG-F(ab')2 Fragment
Reactivity:
Human
Host:
Goat
Clonality:
Polyclonal
Format:
F(ab')2
IsoType:
IgG
Conjugate:
FITC
Purity:
Antigen Affinity Purified
0.5 mg

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Human
Antigen Species
Human
Stock Concentration
0.5 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
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Prod Procedures
Antiserum was solid phase adsorbed to ensure specificity. The antibody to human IgG-F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to fluorescein isothiocyanate (FITC).

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

By immunoelectrophoresis and ELISA this antibody reacts specifically with F(ab')2 fragments of human IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with F(ab')2 fragments of IgG from other species.

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Applications

Not all listed applications have been specifically tested by our laboratory.