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F(ab')2 Goat anti-Mouse IgG-Heavy and Light Chain Antibody DyLight® 650 Conjugated

Bethyl Laboratories®

Bethyl Laboratories® Catalog # A90-138D5

Target:
Mouse IgG-heavy and light chain
Reactivity:
Mouse
Host:
Goat
Clonality:
Polyclonal
Format:
F(ab')2
IsoType:
IgG
Conjugate:
DyLight® 650
Purity:
Antigen Affinity Purified
0.5 mg

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Mouse
Antigen Species
Mouse
Stock Concentration
0.5 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
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Prod Procedures
The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 650.

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

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Applications

Not all listed applications have been specifically tested by our laboratory. DyLight® 650 is excited at 652 (in PBS) and emits at 672 (in PBS). DyLight® 650 replaces DyLight® 649. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.