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F(ab')2 Goat anti-Rat IgG-F(ab')2 Fragment Cross-Adsorbed Antibody FITC Conjugated

Bethyl Laboratories®

Bethyl Laboratories® Catalog # A110-240F

Target:
Rat IgG-F(ab')2 Fragment
Reactivity:
Rat
Host:
Goat
Clonality:
Polyclonal
Format:
F(ab')2
IsoType:
IgG
Conjugate:
FITC
Purity:
Antigen Affinity Purified
0.5 mg

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Rat
Antigen Species
Rat
Minimum Reactivity Species
human,mouse
Stock Concentration
0.5 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
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Prod Procedures
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG-F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. F(ab')2 fragments were conjugated to fluorescein isothiocyanate (FITC).

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

By immunoelectrophoresis and ELISA this antibody reacts specifically with the F(ab')2 of rat IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and mouse F(ab')2 was detected. This antibody may cross react with F(ab')2 fragments of IgG from other species.

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Applications

Not all listed applications have been specifically tested by our laboratory.