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F(ab')2 Goat anti-Rat IgG-Fc Fragment Antibody Alkaline Phosphatase Conjugated

Bethyl Laboratories®

Bethyl Laboratories® Catalog # A110-139AP

Target:
Rat IgG-Fc Fragment
Reactivity:
Rat
Host:
Goat
Clonality:
Polyclonal
Format:
F(ab')2
IsoType:
IgG
Conjugate:
Alkaline Phosphatase
Purity:
Antigen Affinity Purified
0.5 mg

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Rat
Antigen Species
Rat
Stock Concentration
0.5 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 containing 0.2% BSA and 0.09% NaN3
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Prod Procedures
Antiserum was solid phase adsorbed to ensure class specificity. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to alkaline phosphatase.

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

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Applications

Not all listed applications have been specifically tested by our laboratory.