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Goat anti-Human IgM Cross-Adsorbed Antibody DyLight® 594 Conjugated

Bethyl Laboratories®

Bethyl Laboratories® Catalog # A80-200D4

Target:
Human IgM
Reactivity:
Human
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
IsoType:
IgG
Conjugate:
DyLight® 594
Purity:
Antigen Affinity Purified
0.5 mg

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Human
Antigen Species
Human
Minimum Reactivity Species
mouse,rat
Stock Concentration
0.5 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
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Prod Procedures
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human IgM was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgM was detected. This antibody may cross react with IgM from other species.

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Applications

Not all listed applications have been specifically tested by our laboratory. DyLight® 594 is excited at 593 (in PBS) and emits at 618 (in PBS). DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.