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Goat anti-Mouse IgG1 Antibody Alkaline Phosphatase Conjugated

Bethyl Laboratories®

Bethyl Laboratories® Catalog # A90-105AP

Target:
Mouse IgG1
Reactivity:
Mouse
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
IsoType:
IgG
Conjugate:
Alkaline Phosphatase
Purity:
Antigen Affinity Purified
0.5 mg

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Mouse
Antigen Species
Mouse
Stock Concentration
0.5 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 containing 0.2% BSA and 0.09% NaN3
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Prod Procedures
Antiserum was solid phase adsorbed to ensure subclass specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to alkaline phosphatase (alkphos).

Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme:antibody protein ratio is 1:1.

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity to mouse IgA, IgM, IgG2a, IgG2b, IgG2c, IgG3 and IgE is undetectable. Some hybridoma clones may express aberrant immunoglobulin-related peptides that are improperly recognized by this antibody.

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Applications

Not all listed applications have been specifically tested by our laboratory.