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Goat anti-Rabbit IgG-heavy and light highly Cross-Adsorbed Antibody HRP Conjugated

Bethyl Laboratories®

Bethyl Laboratories® Catalog # A120-501P

Target:
Rabbit IgG-heavy and light chain
Reactivity:
Rabbit
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
IsoType:
IgG
Conjugate:
HRP
Purity:
Antigen Affinity Purified
1 mg

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Rabbit
Antigen Species
Rabbit
Minimum Reactivity Species
human,mouse
Stock Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
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Prod Procedures
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Eliminate the streptavidin-HRP step required when using biotinylated secondary antibodies. Incubate samples with the Rabbit IgG-heavy and light chain highly cross-adsorbed IHC Antibody for the length of time used with biotinylated secondary antibodies and proceed directly to visualizing with DAB or other HRP substrate.

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Applications

For use with Formalin-Fixed Paraffin-Embedded tissues, frozen sections and cells. IHC validation was performed using Immunohistochemistry Accessory Kit (Cat. # IHC-00101). A120-501P was substituted for IHC-00101d.