Isothermal DNA Amplification for Molecular Diagnostics
Isothermal amplification is a method that enables nucleic acid detection at a fixed temperature, with this feature making isothermal amplification desirable for use in rapid, portable, point-of-care (POC) assays.
There are several different isothermal amplification methods which utilize different enzymes and master mix components for different applications. For example, Loop-mediated isothermal amplification (LAMP) requires Bst polymerase and works at 65 °C to increase specificity; whereas, Whole Genome Amplification (WGA) requires Phi29 Polymerase and works at lower temperatures (30±5°C). All isothermal amplification methods require DNA polymerases with high strand displacement activity, sequence specific primers for specificity to the target gene, single stranded binding proteins, and secondary enzymes.
At Fortis Life Sciences, we develop and manufacture custom enzymes, proteins, and master mixes that meet all your needs for isothermal amplification assay development - whether it be for detecting RNA or DNA from human, animal or environmental samples on a lateral flow strip or using another detection method.
Isothermal DNA Amplification Methods
We have the expertise to custom design isothermal amplification enzymes for common methods, such as LAMP and Whole Genome Amplification (WGA).Loop-Mediated Isothermal Amplification (LAMP)
Loop-mediated isothermal amplification is a method utilizing a single-tube technique at a constant temperature (around 60-65℃) where 4-6 primers and strand displacing enzymes are used to form a loop structure which enables quick amplification of target genes.Whole Genome Amplification (WGA)
Whole Genome Amplification is another isothermal method that is used to produce large quantities of an entire genome of an organism from a limited amount of starting material.
Complete your isothermal amplification assay development with custom LFA Development and Manufacturing.
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