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Purified Dog IgE
Bethyl Laboratories®
Catalog # P115
Target:
Dog IgE
Host:
Dog
Conjugate:
Unconjugated
Purity:
Purified
Unconjugated (100 µg)
Product Details
Specifications
Antigen Species
Dog
Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Production Procedures
Monoclonal canine IgE specific for a nematode antigen was isolated from the cell culture supernatant of a canine x-mouse heterohybridoma cell line using protein A affinity chromatography.
Concentration is 1. 0 mg/ml determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1. 0 mg of IgE.
By SDS-PAGE nonreduced gels show only one band, at 200 kilodaltons (kd), indicating 95% purity, and reduced gels show only two bands, at 75 kd and 25 kd. This protein at concentrations as low as 1 ng/ml is detected by mouse monoclonal Antibodies with biotin, binds to canine mast cells as detected by avidin-phycoerythrin using flow cytometry.
Additional Product Information
IgE monomers contain two heavy chains and two light chains, and are primarily involved in the immune response to parasites, and has been associated with allergies and asthma.
Applications
