System.Dynamic.ExpandoObject
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Purified Human IgG/Lambda, normal serum

Bethyl Laboratories®

Bethyl Laboratories® Catalog # P80-312

Target:
Human IgG/Lambda
Host:
Human
100 mg

For ordering information, see our International Distributors

Product Details

Specifications
Antigen Species
Human
Stock Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
2 years from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.1% Sodium Azide
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Prod Procedures
Purified normal human IgG is isolated using fractionation and DEAE chromatography. IgG with lambda chains was purified using affinity chromatography.

Protein concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

By immunoelectrophoresis the IgG/lambda was shown to 1) react with antiserum specific for human IgG-Fc and lambda chains, 2) not react with antiserum specific for IgA, IgM or kappa chain and 3) produce a single precipitin arc with antiserum against human serum identical to that produced with anti-IgG.

By ELISA the IgG/lambda contains less than 0.1% of IgA, IgM and Kappa.

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Applications

Used as a positive control in immunoassays. Serum or plasma from donor has been tested and found negative for HIV-1 antigen, antibodies for HIV and HCV, and non-reactive for HBsAg, HIV-1 RNA, and HCV RNA. Not tested for Anti-HBc. HANDLE AS IF CAPABLE OF TRANSMITTING INFECTIOUS AGENTS.

Citations (2)