E. coli UNG
Empirical Bioscience®
Catalog #
Applications:
Carryover Prevention
or
5,000 Units
25,000 Units
$257.40
$1,188.03
Product Details
This enzyme is a uracil N-glycosylase (UNG) obtained from the bacterium Esherichia coli. UNG enzymes specifically hydrolyze the N-glycosidic bond of uracil residues in ss/dsDNA molecules leaving an abasic site in the strand but will not react with RNA. The optimal temperature range for E. coli UNG activity is from 30-40 °C. The enzyme is irreversibly inactivated after 10 minutes of incubation at temperatures ≥95 °C making it suited for carryover prevention in standard PCR and qPCR applications. It is active in most standard reaction buffers used for derivatives of Taq and Pfu DNA polymerases.