Goat anti-Myc Tag Antibody Affinity Purified
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Goats were immunized with a synthetic peptide representing amino acid residues 410-419 (EQKLISEEDL) of human myc conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to horseradish peroxidase (HRP).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1. Goats were immunized with c-myc (EQKLISEEDL) conjugated to KLH.
Antibody was affinity purified using the peptide immobilized on solid support. Goat anti-c-myc affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method. Final concentration is 0.1 mg antibody in 0.4 ml of 50% slurry containing 0.2 ml agarose.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4, before coupling to agarose beads.
Additional Product Information
The Myc epitope tag is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized c-myc epitope represents amino acid residues 410-419, EQKLISEEDL, of human c-myc.
Alternate Names
avian myelocytomatosis viral oncogene homolog; bHLHe39; class E basic helix-loop-helix protein 39; c-Myc; MRTL; myc proto-oncogene protein; MYCC; myc-related translation/localization regulatory factor; proto-oncogene c-Myc; transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog
Applications
Not all listed applications have been specifically tested by our laboratory.
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots are performed using Donkey anti-Goat IgG Heavy and Light Chain Antibody (Cat. No. A50-101P).