Rabbit anti-14-3-3 Sigma IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00346 maps to a region between residue 198 and 248 of human 14-3-3 sigma using the numbering given in entry NP_006133.1 (GeneID 2810).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to 14-3-3 Sigma immobilized on solid support.
The epitope recognized by IHC-00346-T maps to a region between residue 198 and 248 of human 14-3-3 sigma using the numbering given in entry NP_006133.1 (GeneID 2810).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
14-3-3 sigma was identified as an epithelial cell marker. It is part of the 14-3-3 family of proteins in which seven isoforms have been identified: beta, zeta, gamma, eta, epsilon, tau, and sigma. 14-3-3 proteins function as adaptors that bind with a number of partners to mediate various signaling pathways. 14-3-3 sigma is also called stratifin or HME1 and appears to function as a tumor suppressor whose expression can be downregulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis.
Alternate Names
14-3-3 protein sigma; epithelial cell marker protein 1; Stratifin; YWHAS
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.