Rabbit anti-14-3-3 theta Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A303-146A maps to a region between residue 195 and 245 of human 14-3-3 protein theta using the numbering given in entry NP_006817.1 (GeneID 10971).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to 14-3-3 theta immobilized on solid support.
The epitope recognized by A303-146A-T maps to a region between residue 195 and 245 of human 14-3-3 protein theta using the numbering given in entry NP_006817.1 (GeneID 10971).
Additional Product Information
14-3-3 theta is a member of the 14-3-3 family of proteins which serve as adapter proteins in specialized signal transduction pathways. There are 7 members of the 14-3-3 family of proteins: beta, gamma, epsilon, zeta, eta, theta, and sigma.
Alternate Names
14-3-3; 14-3-3 protein tau; 14-3-3 protein T-cell; 14-3-3 protein theta; 14-3-3 theta; 1C5; HS1; Protein HS1; protein, theta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.