Rabbit anti-A-RAF Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A303-104A maps to a region between residue 150 and 200 of human A-Raf Proto-Oncogene Serine/Threonine-Protein Kinase using the numbering given in entry NP_001645.1 (GeneID 369).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to A-RAF immobilized on solid support.
The epitope recognized by A303-104A-T maps to a region between residue 150 and 200 of human A-Raf Proto-Oncogene Serine/Threonine-Protein Kinase using the numbering given in entry NP_001645.1 (GeneID 369).
Additional Product Information
A-RAF is a member of the RAF family of serine/threonine kinases. In mammals, there are three members: A-,B-, and C-RAF. A-RAF plays a role in the transduction of cellular signals that promote cell growth and development.
Alternate Names
A-RAF; A-Raf proto-oncogene serine/threonine-protein kinase; ARAF1; Oncogene ARAF1; PKS2; Proto-oncogene A-Raf; proto-oncogene A-Raf-1; proto-oncogene Pks; RAFA1; Ras-binding protein DA-Raf; serine/threonine-protein kinase A-Raf; v-raf murine sarcoma 3611 viral oncogene homolog 1; v-raf murine sarcoma 3611 viral oncogene-like protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.