Bethyl Laboratories
Rabbit anti-AF4 Antibody Affinity Purified
Primary
Catalog #A302-344A
TargetAF4
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
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Product Description
AF4 is a member of the AF4/LAF4/FMR2 (ALF) family of transcription cofactors. It has been identified as part of a translocation [t(4:11)(q21;q23)] found in patients with acute leukemia involving MLL/HRX. AF4 functions as a cofactor of transcriptional elongation and chromatin remodeling.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetAF4
Antigen Species
Human
Gene ID4299
Gene Symbol
AFF1
Gene Name
AF4/FMR2 family member 1
Uniprot IDP51825
Protein Name
AF4/FMR2 family member 1
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to AF4 immobilized on solid support.
The epitope recognized by A302-344A maps to a region between residue 1 and 50 of human proto-oncogene AF4 using the numbering given in entry NP_005926.1 (GeneID 4299).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-344A · Lot 2 (current lot)Open
Product DatasheetPDF · A302-344A · Lot 2 (current lot)Open
Safety Data SheetPDF · A302-344AOpen
Citations / Publications
Services
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