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Rabbit anti-AF9 IHC Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
AF9
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 250 and 300
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (50+ slides)

10 µl (5+ slides)

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Product Details

Specifications
Verified Reactivity
Human
Presumed Reactivity
Ferret, White-tufted-ear marmoset, Monkey, Pig, Naked mole rat, Rabbit, Little brown bat, Gorilla, Chimpanzee, Northern white-cheeked gibbon, Dog, Bovine, Orangutan, Horse
Antigen Species
Human
Stock Concentration
250 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to AF9 immobilized on solid support.

The epitope recognized by IHC-00125-T maps to a region between residues 250 and 300 of human ALL1 Fused Gene from Chromosome 9 Protein (MLLT3, Myeloid/Lymphoid or Mixed-Lineage Leukemia Translocate 3) using the numbering given in entry NP_004520.1 (GeneID 4300).

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Gene & Protein Information

The human AF9 gene is one of the most common fusion partner genes with the MLL (mixed lineage leukemia) gene resulting in AF9/MLL translocations and leukemogenesis. AF9 is highly homologous to the gene product of ENL, another common MLL fusion partner gene. The function of AF9 is not known but through studies of ENL it is proposed to be a nuclear transactivating factor.

4300

MLLT3

MLLT3, super elongation complex subunit

P42568

Protein AF-9

Alternate Names

AF9, ALL1-fused gene from chromosome 9 protein, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to, 3, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3, myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein, myeloid/lymphoid or mixed-lineage leukemia; translocated to 3, protein AF-9, YEATS domain-containing protein 3, YEATS3

Applications

Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections. In some cases, the antibody may be diluted further than indicated.