/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-alpha Actinin 4/ACTN4 Antibody
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Primary Antibodies  

Rabbit anti-alpha Actinin 4/ACTN4 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

alpha Actinin 4/ACTN4

Reactivity:

Human

,

Mouse

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1 and 50

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Bovine,

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Alpha Actinin 4 / ACTN4 is an F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). It is probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). It is involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. It links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). alpha Actinin 4 / ACTN4 may also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O43707].

81

ACTN4

actinin alpha 4

O43707

Alpha-actinin-4

Alternate Names

ACTININ-4; alpha-actinin-4; focal segmental glomerulosclerosis 1; FSGS; FSGS1; non-muscle alpha-actinin 4

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.