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Rabbit anti-AMPK alpha 2 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
AMPK alpha 2
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 350 and 400
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

100 µl (10 blots)

20 µl (2 blots)

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Mouse, Human
Presumed Reactivity
Pig, Orangutan
Antigen Species
Human
Stock Concentration
400 µg/ml
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to AMPK alpha 2 immobilized on solid support.

The epitope recognized by A300-508A-T maps to a region between residues 350 and 400 of human 5'-AMP-activated Protein Kinase, catalytic Alpha-2 chain using the numbering given in entry AAH69823.1 (GeneID 5563).

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Gene & Protein Information

AMP-activated protein kinase (AMPK) is a heterotrimeric protein made up of a catalytic alpha subunit, and a regulatory beta and gamma subunit. There are two distinct genes for the alpha subunit, alpha1 and alpha2. AMPK alpha 2 is the serine/threonine kinase catalytic subunit of the AMPK. AMPK is responsible for regulating fatty acid and cholesterol synthesis. AMPK is activated in response to a wide variety of metabolic stresses and switches off anabolic pathways when ATP levels are depleted.

5563

PRKAA2

protein kinase AMP-activated catalytic subunit alp

P54646

5'-AMP-activated protein kinase catalytic subunit

Alternate Names

5'-AMP-activated protein kinase catalytic subunit alpha-2, 5'-AMP-activated protein kinase, catalytic alpha-2 chain, ACACA kinase, acetyl-CoA carboxylase kinase, AMP-activated protein kinase alpha-2 subunit variant 2, AMP-activated protein kinase alpha-2 subunit variant 3, AMPK, AMPK alpha 2, AMPK subunit alpha-2, AMPK2, AMPKa2, AMPK-alpha-2 chain, HMGCR kinase, hydroxymethylglutaryl-CoA reductase kinase, PRKAA, protein kinase, AMP-activated, alpha 2 catalytic subunit

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-20% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Citations (2)