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Rabbit anti-ANKRD28/PITK Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
ANKRD28/PITK
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 975 and 1025
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

100 µl (10 blots)

20 µl (2 blots)

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Product Details

Specifications
Verified Reactivity
Human
Antigen Species
Human
Stock Concentration
20 µg/ml
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to ANKRD28/PITK immobilized on solid support.

The epitope recognized by A300-974A-T maps to a region between residue 975 and 1025 of human ankyrin repeat domain 28 (phosphatase interactor targeting K protein) using the numbering given in entry NP_056014.2 (GeneID 23243).

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Gene & Protein Information

ANKRD28/PITK is a regulatory subunit of protein phosphatase-1 (PP1) that targets the PP1 catalytic subunit to nuclear foci to dephosphorylate and modulate the transcriptional activity of heterogeneous nuclear ribonucleoprotein K (hnRNPK). ANKRD28/PITK has been shown to be phosphorylated and regulated by calcium/calmodulin-dependent kinase II-delta (CaMKIIdelta) and glycogen synthase kinase-3 (GSK3).

23243

ANKRD28

ankyrin repeat domain 28

O15084

Serine/threonine-protein phosphatase 6 regulatory

Alternate Names

ankyrin repeat domain-containing protein 28, phosphatase interactor targeting K protein, phosphatase interactor targeting protein hnRNP K, PITK, PP6-ARS-A, PPP1R65, protein phosphatase 1, regulatory subunit 65, protein phosphatase 6 ankyrin repeat subunit A, serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A, serine/threonine-protein phosphatase 6 regulatory subunit ARS-A

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-8% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Citations (1)