Rabbit anti-ATG9A Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A305-223A maps to a region between residue 789 to 839 of human Autophagy-related protein 9A using the numbering given in entry Q7Z3C6.3 (GeneID 79065).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ATG9A immobilized on solid support.
The epitope recognized by A305-223A-T maps to a region between residue 789 to 839 of human Autophagy-related protein 9A using the numbering given in entry Q7Z3C6.3 (GeneID 79065).
Additional Product Information
Autophagy-related protein 9A (ATG9A) is involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. It plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. ATG9A cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q7Z3C6].
Alternate Names
APG9 autophagy 9-like 1; APG9L1; APG9-like 1; ATG9 autophagy related 9 homolog A; autophagy 9-like 1 protein; autophagy-related protein 9A; mATG9; MGD3208
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.