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Rabbit anti-ATP6V1H Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
ATP6V1H
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 400 and 450
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Mouse, Human
Presumed Reactivity
Pig, Bovine
Antigen Species
Human
Stock Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to ATP6V1H immobilized on solid support.

The epitope recognized by A304-308A-T maps to a region between residue 400 to 450 of human ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H using the numbering given in entry NP_057025.2 (GeneID 51606).

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

V-type proton ATPase subunit H (ATP6V1H) is a subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity). ATP6V1H is involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9UI12].

51606

ATP6V1H

ATPase H+ transporting V1 subunit H

Q9UI12

V-type proton ATPase subunit H

Alternate Names

ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H, CGI-11, MSTP042, NBP1, nef-binding protein 1, protein VMA13 homolog, SFD, SFDalpha, SFDbeta, vacuolar ATP synthase subunit H, vacuolar ATPase subunit H, vacuolar proton pump subunit H, vacuolar proton pump subunit SFD, V-ATPase 50/57 kDa subunits, V-ATPase subunit H, VMA13, V-type proton ATPase subunit H

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-20% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.