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Rabbit anti-B-Myb IHC Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
B-Myb
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 450 and 500
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (50+ slides)

10 µl (5+ slides)

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Product Details

Specifications
Verified Reactivity
Human
Presumed Reactivity
Ferret, White-tufted-ear marmoset, Crab-eating macaque, Panda, Monkey, African elephant, Pig, Rabbit, Small-eared galago, Guinea pig_10141, Thirteen-lined ground squirrel, Gorilla, Chimpanzee, Northern white-cheeked gibbon, Dog, Bovine, Orangutan, Horse
Antigen Species
Human
Stock Concentration
250 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to B-Myb immobilized on solid support.

The epitope recognized by IHC-00604-T maps to a region between residue 450 and 500 of human MYB-related protein B using the numbering given in entry NP_002457.1 (GeneID 4605).

Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

B-myb is transcription factor involved in multiple cellular processes such as cell survival, proliferation, and differentiation. B-myb was identified in a low-stringency screen of a T-cell cDNA library probed with a C-myb probe. C-myb is a cellular proto-oncogene highly conserved in vertebrates and invertebrates. The chicken c-myb proto-oncogene was the first ortholog discovered. Investigation of the origins of the avian myeloblastosis virus (AMV) led to the discovery that the AMV genome contained transduced sequences from normal chicken cellular DNA that resulted in a viral oncogenic version (v-myb) of the c-myb gene. B-myb is cell cycle regulated and expressed in all proliferating cells. Recent studies show that it is the only myb family member detected in embryonic stem cells and is essential to cell cycle progression and genomic stability.

4605

MYBL2

MYB proto-oncogene like 2

P10244

Myb-related protein B

Alternate Names

BMYB, B-MYB, myb-like protein 2, myb-related protein B, v-myb avian myeloblastosis viral oncogene homolog-like 2, v-myb myeloblastosis viral oncogene homolog-like 2

Applications

Epitope exposure is recommended._x000D_ _x000D_ Epitope exposure with citrate buffer will enhance staining._x000D_ _x000D_ Likely to work with frozen sections._x000D_ _x000D_ In some cases, the antibody may be diluted further than indicated.