Bethyl Laboratories
Rabbit anti-BORG5 Antibody Affinity Purified
Primary
Catalog #A302-381A
TargetBORG5
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
BORG5 (binder of Rho GTPases 5) is part of a family of proteins that function as negative regulators of CDC42 Rho GTPase signaling. The Borg family of proteins are also known as CEPs (Cdc effector proteins) and have been shown to act downstream of Cdc42 to influence the assembly of actin filaments and changes in cell shape. BORG5 plays a role in mannose receptor-mediated phagocytosis of unopsonized Pneumocystis organisms by alveolar macrophages.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 150 and 200
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetBORG5
Antigen Species
Human
Gene ID11135
Gene Symbol
CDC42EP1
Gene Name
CDC42 effector protein 1
Uniprot IDQ00587
Protein Name
Cdc42 effector protein 1
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to BORG5 immobilized on solid support.
The epitope recognized by A302-381A maps to a region between residue 150 and 200 of human binder of Rho GTPases 5 using the numbering given in entry NP_689449.1 (GeneID 11135).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-381A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-381A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-381AOpen
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