Rabbit anti-BORG5 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-381A-T maps to a region between residue 150 and 200 of human binder of Rho GTPases 5 using the numbering given in entry NP_689449.1 (GeneID 11135).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to BORG5 immobilized on solid support.
The epitope recognized by A302-381A maps to a region between residue 150 and 200 of human binder of Rho GTPases 5 using the numbering given in entry NP_689449.1 (GeneID 11135).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
BORG5 (binder of Rho GTPases 5) is part of a family of proteins that function as negative regulators of CDC42 Rho GTPase signaling. The Borg family of proteins are also known as CEPs (Cdc effector proteins) and have been shown to act downstream of Cdc42 to influence the assembly of actin filaments and changes in cell shape. BORG5 plays a role in mannose receptor-mediated phagocytosis of unopsonized Pneumocystis organisms by alveolar macrophages.
Alternate Names
55 kDa bone marrow stromal/endothelial cell protein; binder of Rho GTPases 5; BORG5; CDC42 effector protein (Rho GTPase binding) 1; cdc42 effector protein 1; CEP1; MSE55; serum constituent protein; serum protein MSE55
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.