Bethyl Laboratories
Rabbit anti-BRF1 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00270
TargetBRF1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
BRF1 is one of three subunits of the RNA polymerase III transcription factor complex, TFIIIB. TFIIIB regulates RNA Polymerase III transcription which manufactures tRNAs, 5S ribosomal RNA and several other untranslated RNAs.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 627 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetBRF1
Antigen Species
Human
Gene ID2972
Gene Symbol
BRF1
Gene Name
BRF1, RNA polymerase III transcription initiation factor subunit
Uniprot IDQ92994
Protein Name
Transcription factor IIIB 90 kDa subunit
Alternate Names
B-related factor 1 • BRF • BRF-1 • BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB • BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit • CFDS • epididymis secretory sperm binding protein Li 76p • general transcription factor IIIB, 90kD subunit • GTF3B • hBRF • HEL-S-76p • hTFIIIB90 • TAF3B2 • TAF3C • TAFIII90 • TATA box binding protein (TBP)-associated factor 3C • TATA box-binding protein-associated factor, RNA polymerase III, subunit 2 • TBP - associated factor, RNA polymerase III, 90kD • TF3B90 • TFIIIB90 • transcription factor IIIB 90 kDa subunit
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to BRF1 immobilized on solid support.
The epitope recognized by IHC-00270 maps to a region between residue 627 and 677 of human B - related factor 1 using the numbering given in entry NP_001510.2 (GeneID 2972).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00270Open
Product DatasheetPDF · IHC-00270 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00270 · Lot 1 (current lot)Open
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