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Rabbit anti-CASC3 IHC Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
CASC3
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 653 and 703
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (50+ slides)

10 µl (5+ slides)

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Product Details

Specifications
Verified Reactivity
Human
Presumed Reactivity
White-tufted-ear marmoset, Tasmanian devil, Crab-eating macaque, Panda, Monkey, African elephant, Pig, Rabbit, Little brown bat, Thirteen-lined ground squirrel, Gray short-tailed opossum, Gorilla, Chimpanzee, Northern white-cheeked gibbon, Dog, Bovine, Orangutan, Horse
Antigen Species
Human
Stock Concentration
250 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to CASC3 immobilized on solid support.

The epitope recognized by IHC-00690-T maps to a region between residue 653 and 703 of human cancer susceptibility candidate 3 using the numbering given in entry NP_031385.2 (GeneID 22794).

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

Cancer susceptibility candidate gene 3 protein (CASC3) is a core component of the exon junction complex (EJC), a protein complex that is deposited on spliced mRNAs at exon-exon junctions and functions in nonsense-mediated mRNA decay (NMD). The encoded protein binds RNA and interacts with two other EJC core components. CASC3 is predominantly located in the cytoplasm, but shuttles into the nucleus where it localizes to nuclear speckles [taken from NCBI Entrez Gene (GeneID: 22794)].

22794

CASC3

CASC3, exon junction complex subunit

O15234

Protein CASC3

Alternate Names

barents, ZBT, Zcancer susceptibility 3, cancer susceptibility candidate 3, cancer susceptibility candidate gene 3 protein, metastatic lymph node 51, metastatic lymph node gene 51 protein, MLN 51, MLN51, protein barents, Zprotein CASC3

Applications

Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections. In some cases, the antibody may be diluted further than indicated.