Rabbit anti-CASC5 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-804A-T maps to a region between residue 250 and 300 of human Cancer Susceptibility Candidate 5 using the numbering given in entry NP_733468.2 (GeneID 57082).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CASC5 immobilized on solid support.
The epitope recognized by A300-804A maps to a region between residue 250 and 300 of human Cancer Susceptibility Candidate 5 using the numbering given in entry NP_733468.2 (GeneID 57082).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Cancer susceptibility candidate gene 5 protein (CASC5) is predominantly expressed in testis and widely expressed in various human tumor cell lines and primary tumors from various tissues and organs. CASC5 is most frequently expressed in lung cancer and has been identified as a fusion-partner of the mixed lineage leukemia (MLL) gene, a gene often activated by chromosomal translocation in leukemias. CASC5 has also been identified as a component of the Mis12 kinetochore complex that is required for proper chromosome segregation.
Alternate Names
AF15Q14; ALL1-fused gene from chromosome 15q14 protein; Blinkin; blinkin, bub-linking kinetochore protein; Bub-linking kinetochore protein; cancer susceptibility candidate 5; cancer susceptibility candidate gene 5 protein; cancer/testis antigen 29; CASC5; CT29; D40; hKNL-1; hSpc105; kinetochore null 1 homolog; kinetochore scaffold 1; kinetochore-null protein 1; MCPH4; microcephaly, primary autosomal recessive 4; PPP1R55; Protein CASC5; Protein D40/AF15q14; protein phosphatase 1, regulatory subunit 55; Spc7
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.