Bethyl Laboratories
Rabbit anti-Cbl-b Antibody Affinity Purified
Primary
Catalog #A302-903A
TargetCbl-b
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
Image 1 of 1
local_shipping
Order within 15 hours and 27 minutes for shipment by Tuesday, November 17
Product Description
Cbl-b is an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Cbl-b negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q13191].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 932 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCbl-b
Antigen Species
Human
Gene ID868
Gene Symbol
CBLB
Gene Name
Cbl proto-oncogene B
Uniprot IDQ13191
Protein Name
E3 ubiquitin-protein ligase CBL-B
Alternate Names
Cas-Br-M (murine) ecotropic retroviral transforming sequence b • casitas B-lineage lymphoma proto-oncogene b • Cbl proto-oncogene B, E3 ubiquitin protein ligase • Cbl proto-oncogene, E3 ubiquitin protein ligase B • Cbl-b • E3 ubiquitin-protein ligase CBL-B • Nbla00127 • RING finger protein 56 • RING-type E3 ubiquitin transferase CBL-B • RNF56 • SH3-binding protein CBL-B • signal transduction protein CBL-B
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Cbl-b immobilized on solid support.
The epitope recognized by A302-903A maps to a region between residue 932 and 982 of human Cas-Br-M (murine) Ecotropic Retroviral Transforming Sequence b using the numbering given in entry NP_733762.2 (GeneID 868).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-903A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-903A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-903AOpen
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1