/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-CD8 alpha Recombinant Monoclonal Antibody [BLR173J]
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Primary Antibodies  

Rabbit anti-CD8 alpha Recombinant Monoclonal Antibody [BLR173J]

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

CD8 alpha

Reactivity:

Mouse

Applications:

IHC

,

IP

,

WB

Platforms:

COMET™

,

PhenoImager™ HT

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR173J

Format:

Whole IgG

Immunogen:

Residues 28-194 (ECD)

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

$127.00 $794.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Mouse

Antigen Species

Mouse

Concentration
500 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


Request Formulation Change
Production Procedures

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a recombinant protein representing the extracellular domain of mouse T-cell surface glycoprotein CD8 alpha (residues 28-194, NP_001074579.1).

Country of Origin

USA

Additional Product Information

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain [taken from NCBI Entrez Gene (Gene ID: 925)].

12525

Cd8a

CD8 antigen, alpha chain

P01731

T-cell surface glycoprotein CD8 alpha chain

Alternate Names

BB154331; CD antigen CD8a; Ly-2; Ly-35; Ly-B; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.