Rabbit anti-CDK2 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A301-812A maps to a region between residue 248 and 298 of human cyclin-dependent kinase 2 using the numbering given in entry NP_001789.2 (GeneID 1017).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CDK2 immobilized on solid support.
The epitope recognized by A301-812A-T maps to a region between residue 248 and 298 of human cyclin-dependent kinase 2 using the numbering given in entry NP_001789.2 (GeneID 1017).
Additional Product Information
Cyclin-dependent kinase 2 (CDK2) is a member of the CDK family of protein kinases that regulate cell cycle progression. CDK2 associates with cyclin E or cyclin A to drive the cell cycle through G1- and S-phase. Substrates of CDK2 include pRb, p53, p27, E2F, Brca1, CDK7, B-Myb, PP1 alpha, RNA Pol II CTD, axin, p220 NPAT, ribonucleotide reductase M2, Myo-D, MARCKS, HsCdc6, and CP110.
Alternate Names
cdc2-related protein kinase; CDKN2; cell division protein kinase 2; cyclin-dependent kinase 2; p33 protein kinase; p33(CDK2)
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.