Rabbit anti-CDK5RAP3 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
CDK5RAP3 (CDK5 regulatory subunit-associated protein 3) was identified as a protein that associates with the activating subunit of Cdk5, p25NCK5A. Cdk5 is a brain specific cyclin-dependent kinase important to mammalian brain development; however CDK5RAP3 appears to be widely expressed in human tissues. CDK5RAP3 appears to exhibit functions that extend beyond its association with Cdk5. CDK5RAP3 is involved in modulating the G2/M DNA damage checkpoint and can associate with and regulate the Cdk1-cyclin B1 complex. CDK5RAP3 has also been demonstrated to interact with and regulate the tumor suppressor ARF (alternative reading frame) to further play a role in cell cycle progression.
Alternate Names
C53; CDK5 activator-binding protein C53; CDK5 regulatory subunit associated protein IC53-2; CDK5 regulatory subunit-associated protein 3; HSF-27; IC53; ischemic heart CDK5 activator-binding protein C53; LXXLL/leucine-zipper-containing ARFbinding protein; LXXLL/leucine-zipper-containing ARF-binding protein; LZAP; MST016; OK/SW-cl.114; PP1553; Protein HSF-27
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.