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Rabbit anti-CENP-F/Mitosin Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
CENP-F/Mitosin
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 2350 and 2400
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

100 µl (10 blots)

20 µl (2 blots)

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Product Details

Specifications
Verified Reactivity
Human
Antigen Species
Human
Stock Concentration
40 µg/ml
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to CENP-F/Mitosin immobilized on solid support.

The epitope recognized by A301-617A-T maps to a region between residue 2350 and 2400 of human centromere protein F (Mitosin) using the numbering given in entry AAA82889.1 (GeneID 1063).

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Gene & Protein Information

Centromere protein F (CENP-F) is a microtubule binding protein and component of the kinetochore. It is essential for kinetochore attachment, chromosome alignment, and the spindle checkpoint. CENP-F appears to be important to the association of the NudE-related proteins Ndel1 and Nde1 to the Lis1/dynein motor complex and their role in kinetochore function. CENP-F is a farnesylated protein and the farnesylation of CENP-F appears to be central to its role in mediating kinetochore-microtubule interactions as well as its degradation after mitosis. Recently, CENP-F expression has been correlated with markers of poor outcome in breast cancer.

1063

CENPF

centromere protein F

P49454

Centromere protein F

Alternate Names

AH antigen, cell-cycle-dependent 350K nuclear protein, CENF, CENP-F, CENP-F kinetochore protein, centromere protein F, centromere protein F, 350/400kDa, CILD31, hcp-1, kinetochore protein CENPF, mitosin, PRO1779, STROMS

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 3-8% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 3-8% SDS-PAGE.

Citations (3)