Rabbit anti-CIZ Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A303-251A maps to a region between residue 527 and 577 of human Cas-Interacting Zinc Finger Protein using the numbering given in entry NP_001129206.1 (GeneID 171017).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CIZ immobilized on solid support.
The epitope recognized by A303-251A-T maps to a region between residue 527 and 577 of human Cas-Interacting Zinc Finger Protein using the numbering given in entry NP_001129206.1 (GeneID 171017).
Additional Product Information
CAS-interacting zinc finger protein (CIZ) is also known as nuclear matrix protein 4 (NMP4) and the gene name zinc finger protein 384 (ZNF384). CIZ is a C2H2-type zinc finger protein, which may function as a transcription factor. CIZ appears to bind and regulate the promoters of the extracellular matrix genes MMP1, MMP3, MMP7 and COL1A1. Studies in mouse suggest that nuclear matrix transcription factors (NP/NMP4) may be part of a general mechanical pathway that couples cell construction and function during extracellular matrix remodeling [taken from NCBI Entrez Gene (Gene ID: 171017)].
Alternate Names
CAG repeat protein 1; CAGH1; CAGH1A; Cas-interacting zinc finger protein; CI; ZERDA2; expanded repeat domain, CAG/CTG 2; NMP4; NP; Nuclear matrix protein 4; nuclear matrix transcription factor 4; TNRC1; trinucleotide repeat-containing gene 1 protein; zinc finger protein 384
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.