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Rabbit anti-CLP36 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
CLP36
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 225 and 275
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Bovine
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to CLP36 immobilized on solid support.

The epitope recognized by A301-956A-T maps to a region between residue 225 and 275 of human CLP36 using the numbering given in entry NP_066272.1 (GeneID 9124).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to CLP36 immobilized on solid support.

The epitope recognized by A301-956A maps to a region between residue 225 and 275 of human CLP36 using the numbering given in entry NP_066272.1 (GeneID 9124).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

CLP36 is a PDZ-domain and LIM-domain containing protein that associates with actin filaments and stress fibers via alpha-actinin. CLP36 has been show to be critical to stress fiber formation and focal adhesions.

9124
PDLIM1
PDZ and LIM domain 1
O00151
PDZ and LIM domain protein 1

Alternate Names

carboxyl terminal LIM domain protein 1; CLIM1; CLP36; CLP-36; C-terminal LIM domain protein 1; elfin; epididymis secretory protein Li 112; hCLIM1; HEL-S-112; LIM domain protein CLP-36; PDZ and LIM domain protein 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.