Rabbit anti-CLP36 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A301-956A-T maps to a region between residue 225 and 275 of human CLP36 using the numbering given in entry NP_066272.1 (GeneID 9124).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CLP36 immobilized on solid support.
The epitope recognized by A301-956A maps to a region between residue 225 and 275 of human CLP36 using the numbering given in entry NP_066272.1 (GeneID 9124).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
CLP36 is a PDZ-domain and LIM-domain containing protein that associates with actin filaments and stress fibers via alpha-actinin. CLP36 has been show to be critical to stress fiber formation and focal adhesions.
Alternate Names
carboxyl terminal LIM domain protein 1; CLIM1; CLP36; CLP-36; C-terminal LIM domain protein 1; elfin; epididymis secretory protein Li 112; hCLIM1; HEL-S-112; LIM domain protein CLP-36; PDZ and LIM domain protein 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.