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Rabbit anti-CoCoA Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
CoCoA
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 641 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
Rat
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to CoCoA immobilized on solid support.

The epitope recognized by A300-818A maps to a region between residue 650 and the C-terminus (residue 691) of human Coiled-Coil Transcriptional Coactivator 1 using the numbering given in entry NP_065949.1 (GeneID 57658).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to CoCoA immobilized on solid support.

The epitope recognized by A300-818A-T maps to a region between residue 650 and the C-terminus (residue 691) of human Coiled-Coil Transcriptional Coactivator 1 using the numbering given in entry NP_065949.1 (GeneID 57658).
Country of Origin
USA

Additional Product Information

CoCoA (coiled-coil coactivator) functions as a secondary coactivator for nuclear receptors and the LEF1/beta-catenin transcription factor. CoCoA is recruited to DNA bound nuclear receptors or factors by one of three p160 coactivator proteins that act as the primary coactivator. Through this interaction, CoCoA is able to contribute to transcriptional activation of target genes.

57658
CALCOCO1
calcium binding and coiled-coil domain 1
Q9P1Z2
Calcium-binding and coiled-coil domain-containing protein 1

Alternate Names

calcium-binding and coiled-coil domain-containing protein 1; calphoglin; Cocoa; coiled-coil coactivator protein; coiled-coil leucine zipper coactivator 1; inorganic pyrophosphatase activator; PP13275; sarcoma antigen NY-SAR-3

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.