Rabbit anti-CSK Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-231A maps to a region between residue 400 to 450 of human C-src Tyrosine Kinase using the numbering given in entry NP_004374.1 (GeneID 1445).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CSK immobilized on solid support.
The epitope recognized by A304-231A-T maps to a region between residue 400 to 450 of human C-src Tyrosine Kinase using the numbering given in entry NP_004374.1 (GeneID 1445).
Additional Product Information
C-Src kinase (CSK) is a non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. CSK phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. CSK suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P41240].
Alternate Names
CSK, non-receptor tyrosine kinase; C-Src kinase; c-src tyrosine kinase; protein-tyrosine kinase CYL; tyrosine-protein kinase CSK
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.