NEW!

Rabbit anti-Cul3 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
Cul3
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 675 and 725
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
X. laevis, X. tropicalis, Rat
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Request Formulation Change
Production Procedures
Antibody was affinity purified using an epitope specific to Cul3 immobilized on solid support.

The epitope recognized by A301-109A-T maps to a region between residue 650 and 700 of human cullin 3 using the numbering given in entry NP_003581.1 (GeneID 8452).
Antibody was affinity purified using an epitope specific to Cul3 immobilized on solid support.

The epitope recognized by A301-109A maps to a region between residue 650 and 700 of human cullin 3 using the numbering given in entry NP_003581.1 (GeneID 8452).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

Cul3 is a member of the cullin group of proteins and functions as a scaffold to bring together ubiquitin E3 ligases, E2 conjugating enzyme, and substrate proteins to mediate the ubiquitination of substrate proteins. Cul3 and its associated ubiquitin ligase activity are essential for mitotic division and required for correct alignment of chromosomes and the completion of cytokinesis. Aurora B is a substrate of Cul3-based E3 ubiquitin ligase activity.

8452
CUL3
cullin 3
Q13618
Cullin-3

Alternate Names

CUL-3; cullin-3; PHA2E

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.