Rabbit anti-Cyclin C Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A301-989A maps to a region between residue 233 and 283 of human cyclin C using the numbering given in entry NP_005181.2 (GeneID 892).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Cyclin C immobilized on solid support.
The epitope recognized by A301-989A-T maps to a region between residue 233 and 283 of human cyclin C using the numbering given in entry NP_005181.2 (GeneID 892).
Additional Product Information
Cyclin C is a member of the cyclin family of regulatory proteins that associate with and activate cyclin-dependent kinases (CDKs) to regulate cell cycle progression. Cyclins exhibit periodicity in their expression and activity which is regulated transcriptionally and post-transcriptionally via phosphorylation and ubiquitination. Cyclin C associates with cyclin-dependent kinase 8 (CDK8). It is also a component of the Mediator complex, a coactivator complex that provides a scaffold to link gene-specific transcription factors to RNA polymerase II and the general transcription machinery.
Alternate Names
CycC; cyclin-C; hSRB11; SRB11; SRB11 homolog
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.