Bethyl Laboratories
Rabbit anti-DBC1/p30 DBC Antibody Affinity Purified
Primary
Catalog #A300-434A
TargetDBC1/p30 DBC
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
The DBC1 (Deleted in Breast Cancer Gene 1 protein) gene is located in a region frequently deleted in breast cancers. In its uncleaved form, DBC1 is localized to the nucleus. Upon cleavage by caspase activity, DBC1 becomes localized to the cytoplasm and functions to promote apoptosis. DBC1 has also been found to bind directly to the estrogen receptor alpha (ERalpha) hormone binding domain to modulate ERalpha expression and mediate hormone-independent breast cancer cell survival.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 873 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetDBC1/p30 DBC
Antigen Species
Human
Gene ID57805
Gene Symbol
CCAR2
Gene Name
cell cycle and apoptosis regulator 2
Uniprot IDQ8N163
Protein Name
Cell cycle and apoptosis regulator protein 2
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DBC1 immobilized on solid support.
The epitope recognized by A300-433A maps to a region between residue 875 and the C-terminus (residue 923) of human Deleted in Breast Cancer 1 using the numbering given in TrEMBL entry Q6P0Q9 (GeneID 57805).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:10,000
Documents
Product DatasheetPDF · A300-434A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-434A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-434AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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