Rabbit anti-DCK Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A304-266A maps to a region between residue 210 to 260 of human Deoxycytidine Kinase using the numbering given in entry NP_000779.1 (GeneID 1633).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DCK immobilized on solid support.
The epitope recognized by A304-266A-T maps to a region between residue 210 to 260 of human Deoxycytidine Kinase using the numbering given in entry NP_000779.1 (GeneID 1633).
Additional Product Information
Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivitiy [taken from NCBI Entrez Gene (Gene ID: 1633)].
Alternate Names
dCK; deoxycytidine kinase; deoxynucleoside kinase
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.