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Rabbit anti-DDX27 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

DDX27

Reactivity:

Human

,

Mouse

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 700 and 750

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (50+ slides)

10 µl (5+ slides)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Monkey

Antigen Species

Human

Concentration
250 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to DDX27 immobilized on solid support.

The epitope recognized by IHC-00634 maps to a region between residue 700 and 750 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 using the numbering given in entry NP_060365.6 (GeneID 55661).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to DDX27 immobilized on solid support.

The epitope recognized by IHC-00634-T maps to a region between residue 700 and 750 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 using the numbering given in entry NP_060365.6 (GeneID 55661).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

DDX27 (DEAD-box protein 27) is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. The function of DDX27 has not been characterized.

55661

DDX27

DEAD-box helicase 27

Q96GQ7

Probable ATP-dependent RNA helicase DDX27

Alternate Names

DEAD (Asp-Glu-Ala-Asp) box polypeptide 27; DEAD box protein 27; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 27; Deficiency of ribosomal subunits protein 1 homolog; dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241; probable ATP-dependent RNA helicase DDX27; RHLP; RNA helicase-like protein

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.